Table of Contents

1. Introduction
2. Principle of Dengue NS1 Rapid Test
3. Test Procedure
4. Interpretation of Results
5. QL Biotech Company Solutions
6. Efficacy and Accuracy
7. References

Introduction

The Dengue NS1 Rapid Test is a critical tool in the early diagnosis of dengue virus infections. Utilizing the detection of the non-structural protein 1 (NS1), the test allows for quick identification of the virus, often before antibodies appear. This article examines how this rapid diagnostic test operates, its effectiveness, and available solutions offered by QL Biotech Company.

Principle of Dengue NS1 Rapid Test

The Dengue NS1 Rapid Test is based on the immunochromatographic assay principle. The test detects the NS1 antigen, a protein released in the body by the dengue virus. It is designed to capture NS1 antigen using specific antibodies, which are immobilized on the test strip. The presence of the antigen is visually represented by a colored line on the test strip.

Test Procedure

1. Sample Collection: A blood, serum, or plasma sample is collected from the patient.
2. Application: The sample is applied to the designated area on the test strip.
3. Buffer Addition: A buffer solution is added to facilitate the flow of the sample along the strip.
4. Development: The test strip is allowed to stand for a specified time, usually 15-20 minutes, for the sample to migrate and interact with the reagents.

Interpretation of Results

Positive Result: A test is considered positive when two colored lines appear: one in the test line region (T) and another in the control line region (C). This indicates the presence of the dengue NS1 antigen.

Negative Result: A negative result is indicated by a single colored line in the control line region (C) with no line in the test line region (T), indicating no presence of the NS1 antigen.

Invalid Result: The absence of a line in the control region (C) suggests an invalid test, necessitating a repeat with a new test device.

QL Biotech Company Solutions

QL Biotech Company provides a range of rapid test kits including their advanced Dengue NS1 Rapid Test solutions. Key features include:

• High Sensitivity: Detects NS1 antigen with a sensitivity of up to 95% within the first three days of symptom onset.
• Specificity: Achieves specificity greater than 98%, reducing false positives.
• Ease of Use: Designed for minimal training, the test can be administered in clinical and field settings.
• Comprehensive Solutions: Kits include controls and necessary reagents for testing, ensuring reliable results.

Efficacy and Accuracy

The efficacy and accuracy of the Dengue NS1 Rapid Test depend on several parameters such as sample quality, timing of the test post-infection, and adherence to protocols. Studies have shown that the test's sensitivity is highest during the early phase of the infection (days 1-3), where NS1 antigens are most concentrated. The accuracy rate can reach up to 98% under optimal conditions.

References

1. WHO. Dengue: Guidelines for Diagnosis, Treatment, Prevention, and Control. Geneva: World Health Organization, 2009.
2. Wei, H., et al. Evaluation of the Dengue NS1 Rapid Test and Elisa for Early Diagnosis of Dengue Virus Infection. Clinical and Vaccine Immunology, vol. 19, no. 4, 2012, pp. 554-557.
3. QL Biotech Company, Product Information Sheet: Dengue Ns1 Rapid Test Kit.
4. Lam, P.K., et al. Clinical Evaluation of a Rapid Diagnostic Test for Detection of the Dengue NS1 Antigen in Human Serum. The American Journal of Tropical Medicine and Hygiene, vol. 97, no. 2, 2017, pp. 424-428.