Table of Contents

1. Introduction
2. Principles of Ultrasonic cell dispersion
3. Parameters Influencing Ultrasonic Cell Dispersion
4. Powersonic Company Solutions
5. References

Introduction

Ultrasonic cell dispersion is a widely used technique to disintegrate cells for various applications, ranging from biological research to industrial processing. The method leverages high-frequency sound waves to disrupt cellular structures, facilitating the release of cellular components for further analysis or use.

Principles of Ultrasonic Cell Dispersion

Ultrasonic cell dispersion relies on the cavitation phenomenon, where ultrasonic waves create microbubbles in a liquid medium. As these bubbles collapse, they generate intense shear forces and high temperature and pressure zones that disrupt cell membranes.

Typically, ultrasonic frequencies between 20 kHz to 40 kHz are employed, with power densities ranging from 10 W/cm² to as high as 100 W/cm² depending on the cell type and desired outcome.

Parameters Influencing Ultrasonic Cell Dispersion

The efficiency of ultrasonic cell dispersion is influenced by several parameters:

• Frequency: Higher frequencies generally produce smaller cavitation bubbles, which can be more effective for disrupting smaller cells or delicate structures.
• Sonication Time: Extended exposure can enhance cell disruption but may also lead to thermal degradation of sensitive biomolecules.
• Temperature: Excessive heat from prolonged sonication can denature proteins, necessitating temperature control.
• Power Density: The intensity of ultrasonic waves directly affects cavitation and, consequently, the efficacy of cell lysis.

Powersonic Company Solutions

Powersonic offers advanced ultrasonic cell dispersers engineered to optimize cell lysis while maintaining sample integrity.

Notable products include:

• Powersonic 4000: Featuring adjustable frequencies from 20 kHz to 40 kHz, with a maximum power output of 150 W, suitable for a wide range of cell types.
• Powersonic 5000 Ultra: This model integrates temperature control mechanisms to prevent thermal damage and allows for precise adjustments in power density and sonication time.

References

1. Kim, S. J., & Lee, K. H. (2021). Applications of Ultrasonics in Cell Lysis. Journal of Acoustic Materials, 12(3), 157-170.
2. Gogate, P. R., & Kabadi, A. M. (2009). A Review on Applications of Cavitation in Bioprocess. Biochemical Engineering Journal, 44(1), 60-72.
3. Powersonic. (2023). Product Catalogue. Powersonic Inc.