Table of Contents

1. Introduction to Rotavirus
2. What is a Rotavirus Test?
3. Rotavirus Test Procedure
4. QL Biotech Company Solutions
5. References

Introduction to Rotavirus

Rotavirus is a contagious virus that causes gastroenteritis, leading to severe diarrhea and vomiting, primarily in infants and young children. According to the World Health Organization, rotavirus is responsible for approximately 215,000 deaths annually in children under five years globally. Early detection and vaccination have been pivotal in reducing the mortality rate.

What is a Rotavirus Test?

A rotavirus test detects the presence of the virus in the stool samples of affected individuals. It is crucial for diagnosing the infection, distinguishing it from other causes of gastroenteritis, and implementing timely medical interventions. The test is particularly important during peak seasons of rotavirus transmission, typically in winter and early spring in temperate climates.

Rotavirus Test Procedure

The rotavirus test includes the following steps:

1. Sample Collection: Stool samples are collected using a clean, dry container. The sample should ideally be collected during the acute phase of diarrhea.
2. Sample Preparation: The stool sample is prepared for analysis, which may involve emulsifying with a buffer solution.
3. Enzyme Immunoassay (EIA): This is the most common method used. It detects rotavirus antigen in stool samples. The presence of a color change indicates a positive result.
4. Polymerase Chain Reaction (PCR): For more detailed analysis, PCR can be employed to detect viral RNA in the stool. It provides high sensitivity and specificity.

Results are typically available within a few hours to a day, depending on the testing facility's capabilities.

QL Biotech Company Solutions

QL Biotech offers innovative diagnostic solutions for rotavirus testing. Their products include:

• QL-Rota Kit: A rapid EIA test that delivers results in under two hours with a sensitivity of 96% and specificity of 98%.
• QL-Rota PCR Kit: Designed for comprehensive viral load analysis, this kit achieves a sensitivity of 99.5% and specificity of 99.8%.

References

• World Health Organization, Rotavirus, WHO Factsheet, 2021.
• Centers for Disease Control and Prevention, Rotavirus Infection, CDC Guidelines, 2022.
• Smith J., et al., Advancements in Rotavirus Diagnostic Testing, Journal of Virology, 2022.
• QL Biotech Company, Product Specifications and Clinical Trials, QL Biotech Publications, 2023.